Growth and Maintenance of Thiobacillus ferrooxidans Cells.
نویسندگان
چکیده
A rapid and sensitive spectrophotometric procedure was developed for monitoring the growth of Thiobacillus ferrooxidans in liquid culture. Values determined for the optical densities at 500 nm of washed T. ferrooxidans cell suspensions were directly proportional to both total cell number and total cell protein concentration and provided an accurate measurement of culture growth rate. The utility of this procedure was demonstrated by conducting physiological studies on the influence of CO(2) and FeSO(4) availability on the growth of T. ferrooxidans. In addition, we describe a procedure for the long-term maintenance of cells T. ferrooxidans that ensures culture purity and genetic stability.
منابع مشابه
BIOOXIDATION OF MOUTEH REFRACTORY GOLD-BEARING CONCENTRATE BY AN ADAPTED THIOBACILLUS FERROOXIDANS
The Mouteh refractory pyrite concentrates at pulp densities of 1.5%, 3%, 4.5% and 6% were treated, using Thiobacillus ferrooxidans DSM 581 and the same bacteria adapted on the Mouteh pyritic concentrate. Compared with a non-adapted culture, use of an adapted inoculum of T. ferrooxidans increased bioleaching rate of iron by a factor of 1.940, 2.011, 1.859 and 1.559 for pulp densities 1.5%, 3%, 4...
متن کاملThiobacillus ferrooxidans, a facultative hydrogen oxidizer.
The type strain (ATCC 23270) and two other strains of Thiobacillus ferrooxidans were able to grow by hydrogen oxidation, a feature not recognized before. When cultivated on H2, a hydrogenase was induced and the strains were less extremely acidophilic than during growth on sulfidic ores. Cells of T. ferrooxidans grown on H2 and on ferrous iron showed 100% DNA homology. Hydrogen oxidation was not...
متن کاملDirect current stimulation of Thiobacillus ferrooxidans bacterial metabolism in a bioelectrical reactor without cation-specific membrane.
A bioelectrical reactor without cation-specific membrane was designed to test effects of direct electrical current on growth of Thiobacillus ferrooxidans bacterium. The results indicated that the cell significantly enhanced the growth of T. ferrooxidans. At a current of 30 mA, the maximum cells density reached 1.39 x 10(9)cells/mL within 84 h, which was 10 times faster than under a conventional...
متن کاملGrowth of Thiobacillus ferrooxidans on Formic Acid.
A variety of acidophilic microorganisms were shown to be capable of oxidizing formate. These included Thiobacillus ferrooxidans ATCC 21834, which, however, could not grow on formate in normal batch cultures. However, the organism could be grown on formate when the substrate supply was growth limiting, e.g., in formate-limited chemostat cultures. The cell densities achieved by the use of the lat...
متن کاملDissolution of sulphur particles by Thiobacillus ferrooxidans: substrate for unattached cells.
The growth of Thiobacillus ferrooxidans on sulphur is known to proceed through the attachment of cells to the sulphur particles. Experiments, However, show that the cells in the liquid phase, which are not attached to the sulphur particles, also grow. It has been shown through the use of a two-compartment membrane reactor that this increase is partially due to the release of ions, corresponding...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 56 9 شماره
صفحات -
تاریخ انتشار 1990